Japanese society of Ova Research

Abstract

Vol.39 No.1

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Gene editing-mediated production of genetically modified livestock
JMOR, 39(1) 17-26, 2022
Fuminori Tanihara1* Maki Hirata2,3 Takeshige Otoi2,3 
1Center for Development of Advanced Medical Technology, Jichi Medical University, Tochigi, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan, 2Bio-Innovation Research Center, Tokushima University, 2272-2 Ishii, ishii, Ishii-cho, Myozai-gun, Tokushima 779-3233, Japan, 3Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Jyosanjima-cho, Tokushima, Tokushima 770-8513, Japan

With the advent of gene editing technologies such as the CRISPR/Cas9 system, it has become possible to introduce desired mutations into an organism's genome. High efficiency gene modification of cells and embryos has greatly reduced the labor and time required to produce gene knock-out and knock-in animals across a wide range of species, including rodent laboratory animals such as mice and rats, as well as livestock such as pigs, cattle, sheep, and goats. Gene editing has been widely used in rodents to elucidate the functions of genes and their relationships to pathogenesis. On the other hand, in livestock, gene editing technology has accelerated the provision of useful traits for industry, and the generation of research models for human medicine, promoting preclinical research into the translation of advanced medical technologies for humans. In this paper, we review the current status of gene editing in livestock, covering studies of genetically modified livestock generated using gene editing technology.

Keywords: ZFN  TALEN  CRISPR/Cas9  Gene editing  Livestock 
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