http://www.jmor.jp/ 日本卵子学会雑誌 http://www.jmor.jp/abstract.php?-DB=jmor&-recid=6357&-action=browse 日本卵子学会 2026-04-01T00:00:00 43 1 1 http://www.jmor.jp/abstract.php?-DB=jmor&-recid=6358&-action=browse 日本卵子学会 2026-04-01T00:00:00 43 1 3 http://www.jmor.jp/abstract.php?-DB=jmor&-recid=6359&-action=browse 日本卵子学会 2026-04-01T00:00:00 in vitro fertilisation are derived from frozen embryos. In addition, the introduction of insurance coverage in 2022 has reduced the financial barriers to infertility treatment, thereby increasing the number of patients and the demand for frozen embryo transfer. On the other hand, the number of embryo transfers covered by insurance is limited. Therefore, to maximise pregnancy outcome per embryo transfer, it is crucial to produce and select high-quality embryos, as well as to apply advanced cryopreservation techniques. Furthermore, maintaining a consistent standard of cryopreservation techniques and ensuring stable outcomes requires a proper quality management system. In light of these circumstances, this article presents the principles and protocols of cryopreservation, highlights the significance of implementing quality management to improve and stabilise clinical outcomes, and introduces specific initiatives undertaken at our clinic.]]> 43 1 13 http://www.jmor.jp/abstract.php?-DB=jmor&-recid=6360&-action=browse 日本卵子学会 2026-04-01T00:00:00 43 1 23 http://www.jmor.jp/abstract.php?-DB=jmor&-recid=6361&-action=browse 日本卵子学会 2026-04-01T00:00:00 43 1 33 http://www.jmor.jp/abstract.php?-DB=jmor&-recid=6362&-action=browse 日本卵子学会 2026-04-01T00:00:00 in vitro fertilization or intracytoplasmic sperm injection). Aneuploidy delayed embryonic development and decreased the iDAScore, irrespective of the fertilization method, and the aneuploidy-induced delay in developmental kinetics of embryos derived from aged patients (≧38 years) was more apparent than that of embryos derived from younger patients (<38 years). Aneuploidy patterns (chromosomal increases or decreases) affected the iDAScore Ver.2.0. Overall, a low iDAScore reflected chromosomal abnormalities, but this was not the case for all types of abnormalities.]]> 43 1 39 http://www.jmor.jp/abstract.php?-DB=jmor&-recid=6363&-action=browse 日本卵子学会 2026-04-01T00:00:00 in vitro embryo production (IVEP). Modified synthetic oviduct fluid without glucose (mSOF) was used for early-cleavage-stage embryos, followed by 5% fetal calf serum (FCS) supplemented with SOF for morula-stage embryos. When SOF+FCS was used for the latter half of culture, the blastocyst development rate was significantly higher (P < 0.05) than in culture in mSOF for the full 7 days after IVF. When TCM199 supplemented with 5.54 mM glucose, FCS, and 0.1 mM β-mercaptoethanol was used for the latter half of the culture there was no significant difference in the blastocyst rate from that of SOF+FCS, but at 6 and 9 days after IVEP, the cell numbers of embryos were significantly greater in the TCM199 supplemented medium. These results show that glucose in the medium is important for embryonic development and that switching to an energy-rich medium at the morula stage is beneficial for the production of high-quality embryos.]]> 43 1 47 Brief Note http://www.jmor.jp/abstract.php?-DB=jmor&-recid=6364&-action=browse 日本卵子学会 2026-04-01T00:00:00 in vitro fertilization (IVF) technology is valuable for the efficient production of quality calves. However, bovine IVF embryos have problems such as low embryonic development and conception rates and frequent occurrence of large-offspring syndrome due to abnormal gene expression. The addition of FLI (FGF2, LIF, and IGF1) to in vitro maturation (IVM) medium improves oocyte maturation. Therefore, this study examined the effects of FLI addition to IVM medium on the maturation of oocytes, and preimplantation development, and the gene expression status of bovine embryos after IVF. There was no significant difference in the nuclear maturation rates of oocytes matured in IVM medium with or without FLI supplementation. The blastocyst developmental rate and mRNA expression levels (FGF4, IGFBP-2, OCT-4, CDX2, and AQP3) showed no differences between maturation in IVM medium with or without FLI supplementation. In conclusion, our results show that different IVM media and the addition of FLI did not affect the nuclear maturation of bovine oocytes, preimplantation development or gene expression status of bovine IVF embryos.]]> 43 1 53 Original http://www.jmor.jp/abstract.php?-DB=jmor&-recid=6365&-action=browse 日本卵子学会 2026-04-01T00:00:00 43 1 61 Original