Abstract
Vol.39 No.1
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Basics of the genome editing in embryos
JMOR, 39(1) 3-8, 2022
The development of genome editing tools, which started in the late 1990s, has been accelerated by the discovery of the CRISPR-Cas9 system in 2012. The CRISPR-Cas9 system is now widely used by individual researchers due to its simplicity and efficiency, and genome-editing animals have been produced by introducing genome-editing tools into fertilized eggs. Using this system, genetically modified small laboratory animals such as mice and rats can be produced in much shorter times than with conventional methods. The same genome-editing tools and methods have been applied to domestic animals, including pigs and cattle, as well as other animals for which genetic modification was difficult in the past. Genome-edited animals are used not only in basic life science research, but also in medicine and industry, and genome-edited food animals are now being distributed. Genome editing technology will become more important and common in the future as it holds great potential for gene therapy in humans. In this article, the basics of genome editing in embryos are reviewed.
