Abstract
Vol.38 No.1
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TE-Biopsy practice at ART Labo
JMOR, 38(1) 19-23, 2021
The current status of clinical programs for preimplantation genetic testing for aneuploidy (PGT-A) in Japan is that authorization from the PGT-A committee of the Japan Obstetrics and Gynecology Society is necessary in order to conduct a PGT-A program. Theoretically, proper biopsy procedures of the trophectoderm cell layer of human blastocyst are crucial for the success of a PGT-A program. Since biopsy is one of the most invasive techniques used at ART Labos, it can affect the survival of the biopsied blastocyst and the results of PGT-A. At our center, the biopsy procedure is performed at the expanded stage of the blastocyst, because plenty of cells are available for biopsy, instead of at the early or full blastocyst stage. Also, artificial shrinkage of the blastocoelic cavity with a laser pulse is always performed prior to biopsy, as well as confirmation of the location of intracellular mass (ICM), in order to avoid ICM cell damage due to opening of the zona with the laser pulse and the biopsy itself. In this article, our practical approach is presented in detail.
