Abstract
Vol.38 No.1
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The tips of Lab work for PGT-A —Ingenuity to accurately analyze using NGS while maintaining minimally invasive biopsy—
JMOR, 38(1) 3-11, 2021
The trophectoderm (TE) biopsy process of collecting some cells from a blastocyst is indispensable in preimplantation genetic testing for aneuploidies (PGT-A). However, TE biopsy is not a little invasive. Therefore, there is a concern that the implantation rate may decrease depending on the blastocyst morphological assessment and the number of TE biopsy cells, but there is also a risk of DNA amplification failure and mosaic diagnosis in a small number of cells. Accordingly, it is necessary to devise a method that produces stable chromosomal analysis results while maintaining high viability of blastocysts after TE biopsy. However, each blastocyst has its own characteristics, and it is necessary to make ingenuity for each embryo, and the embryologists at each clinic are making various efforts to carry out the TE biopsies. In addition, since blastocysts are constantly changing, e.g., contraction and expansion, it is necessary to calmly and patiently perform a TE biopsy. In this paper, we will explain the ingenuity in each process of lab work in PGT-A, based on our background and experience until the novel technique was adopted.
