Abstract
Vol.37 No.2
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Effects of membrane rupture technique in intracytoplasmic sperm injection on fertilization and embryonic development: A comparison of membrane rupture via aspiration and piezo-actuated stimulation
JMOR, 37(2) 121-129, 2020
The piezo-actuated intracytoplasmic sperm injection (ICSI) technique is thought to minimize damage to the oocyte during zona pellucida penetration and to improve fertilization rates. However, only a few studies have described the effects of using a piezo micromanipulator during cell membrane rupture. Therefore, in this study, zona pellucida penetration was performed using a piezo micromanipulator, and cell membrane rupture was performed via aspiration (P-aspiration group) or piezo-actuated stimulation (Piezo group). The blastocyst formation rate was significantly higher in the Piezo group than in the P-aspiration group. Fertilization rates, implantation rates, and miscarriage rates were not different between the two groups. The effects of the procedure after zona pellucida penetration for all cases undergoing ICSI (the distance that the needle was advanced to elicit cell membrane rupture, aspirated volume of cytoplasm, difficulty in sperm discharge and the movement of the needle during sperm discharge) were also evaluated. The aspirated volume of cytoplasm had the strongest effect on embryo development and was significantly smaller in the Piezo group than in the P-aspiration group, resulting in improved embryo development. Thus, our study showed that the piezo-actuated ICSI technique using a piezo micromanipulator for zona pellucida penetration and cell membrane rupture is associated with favorable results.
