Abstract
Vol.37 No.2
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Decision criteria for rescue ICSI reducing the risk of artificial polyspermy —analysis of the extrusion time of the second polar body following conventional IVF measured by a time-lapse imaging system—
JMOR, 37(2) 113-119, 2020
The aim of this study was to establish the decision criteria for rescue ICSI reducing the risk of artificial polyspermy. Oocytes were cultured in a time-lapse incubator 4 hours after conventional IVF (cIVF). Then, the target oocytes for rescue ICSI were selected based on various decision criteria using data obtained by a time-lapse imaging system. The rate of normally fertilized oocytes following cIVF among the selected oocytes was examined for the risk rate of artificial polyspermy. The decision criteria were a combination of the appearance time of the second polar body (4, 5, 6, 7 or 8 h after cIVF) and the ratio of the oocytes extruding the second polar body (0% / 0~30% / less than 100%). When the appearance time of the second polar body was 4 h after cIVF, the risk rate range of artificial polyspermy was 37.4~40.8%; however, this rate decreased to nearly 0% when the appearance time was 8 h after cIVF. On the other hand, the risk rates of artificial polyspermy were not significantly different among the ratio categories of oocytes extruding the second polar body. These results indicate that a longer time to the appearance of the second polar body effectively reduces the risk of artificial polyspermy after rescue ICSI.
