Abstract
Vol.37 No.2
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Evaluation of motility and DNA fragmentation of sperm purified by microfluidics and Migration-Gravity sedimentation
JMOR, 37(2) 107-111, 2020
A typical example of preparation of sperm for in vitro fertilization is density gradient centrifugation. However, there are concerns about sperm DNA fragmentation and cell membrane damage due to the effects of centrifugation and the long preparation time. In order to reduce the effects of physical damage to spermatozoa, we investigated new purification methods which use microfluidics and migration-gravity sedimentation. The sperm motility parameters and DNA fragmentation rates of both methods were significantly improved compared to density gradient centrifugation. In a comparison of the two methods, there was no significant difference in the DNA fragmentation rate, but the migration-gravity sedimentation method collected sperm with higher motility than the microfluidic method. The total number of motile sperm collected was less due to the characteristics of the microfluidic method device. The migration-gravity sedimentation method did not have this shortcoming and can be used for both conventional IVF and ICSI insemination.
