Abstract
Vol.36 No.1
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Original
Effect of phytohemagglutinin (PHA) on nuclear transfer pig embryos in vitro
JMOR, 36(1) 33-43, 2019
This study evaluated the effects of the phytohemagglutinin (PHA) treatment on the developmental competence of parthenotes and somatic cell nuclear-transferred (NT) porcine embryos. In the first part of the experiment, the effects of PHA treatment of different durations (1 mg/ml; 0, 5, or 10 min) on parthenogenetically produced porcine embryos was examined. Although, PHA treatment did not affect the percentage of embryos that cleaved and developed into blastocysts, and the total number of cells per blastocyst, mitochondrial activity was higher (P<0.05) in PHA-treated oocytes. In the second part of the experiment, porcine NT embryos were treated in PHA for 5 min before electrofusion. The cleavage and blastocyst rates of NT embryos treated with PHA were higher (P<0.05). The expression of mitochondrial-related genes in NT embryos was demonstrated for the first time. The expression of ATPase6 and ND1 was higher (P<0.05) in PHA-treated embryos than in the other groups. In conclusion, our experiments clearly demonstrate that pre-fusion treatment in PHA (1 mg/ml; 5 min) improved the development of porcine NT embryos in vitro. The results suggest that PHA enhances embryonic mitochondrial activity, and other energy-related target gene expressions.
