Japanese society of Ova Research

Abstract

Vol.17 No.3

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Research Note
Analysis of Igf2 and Igf2r Genes Expression by RT-PCR in Bovine Fetuses
JMOR, 17(3) 124-127, 2000
DOI: 10.1274/jmor.17.124
Yosuke Kawase1# Ayako Moriki1 Yoshiaki Minato2 Seizo Hamano2 Kiyoshi Matsukawa2 Tomohiro Kono1* 
1Department of Animal Science, Tokyo University of Agriculture, Atsugi-shi, Kanagawa 243-0034, Japan 2Livestock Improvement Association of Japan, Inc., Shinagawa-ku, Tokyo 140-0002, Japan #Present address: CSK Research Park, Inc., Gotemba-shi, Shizuoka 412-8513, Japan

In mammals, insulin-like growth factor 2 (Igf2) plays a key role in mammalian growth and its receptor, Igf2r, which are imprinted and expressed exclusively from paternal and maternal alleles, respectively. Here we examined that expression of Igf2 and Igf2r genes in blastocysts and fetuses ranging from 30 to 180 days of gestation by RT-PCR. The expression of both genes was significantly low at the blastocyst stage, but high expression of both genes was detected throughout the fetal period. These results suggest that Igf2 and Igf2r are important genes for growth regulation in bovine fetuses as well as mice.

Keywords: Igf2  Igf2r  Bovine embryos  Bovine fetuses  Gene expression 
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