Abstract
Vol.15 No.1
- « prev
- ↑Table of Contents
- next »
Effect of Estradiol-17β and Progesterone in Co-Culture with Uterine Epithelium Cells on Rat Embryo Development
JMOR, 15(1) 22-26, 1998
DOI: 10.1274/jmor.15.22
DOI: 10.1274/jmor.15.22
This experiment was designed to evaluate the ability of Estradiol-17β (E2) and progesterone (P4) to promote development of rat blastocysts. Eight-cell rat embryos were co-cultured for 5 days with rat uterine epithelium (UE) cells with inclusion of E2 and/or P4. Embryos were collected from mature Wistar-Imamichi rats. UE cells were recovered from the same washed with modified TCM199 (m 199FCS) and placed in 4-well Multi-dishes. UE cells of monolayers were developed to 70-80% confluence prior to initiation of the embryo culture. Embryos were recovered, selected and randomly placed in one of the four steroid hormone dose concentration media. They were evaluated every 24 h to determine their stage of development. More (p<0.05) embryos were developed to both hatched and post-hatched blastocysts in E2 with a concentration of 3.5 × 10-5, 10-4 M and P4 with a concentration of 3.5 × 10-6, 10-5, 10-4 M. The combination of E2 (3.5 × 10-5M) and P4 (3.5 × 10-6, 10-5, 10-4 M) also improved (p<0.05) both hatched and post-hatched blastocysts. These finding showed that the presence of ovarian steroid hormones in a primary culture of UE cells promotes in vitro development of post-blastocysts in rat embryos.
