Abstract
Vol.14 No.2
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Brief Note
A Trial of Oocyte Maturation and In Vitro Fertilization by Frozen-Thawed Spermatozoa in the Red-Bellied Tamarin (Saguinus labiatus)
JMOR, 14(2) 205-208, 1997
DOI: 10.1274/jmor.14.205
DOI: 10.1274/jmor.14.205
Freezing of epididymal spermatozoa from red-bellied tamarins (Saguinus labiatus) was achieved by using a semen-diluting medium called TTE (Tes, Tris, egg-yolk base) containing 5% glycerol. When the frozen spermatozoa were suspended and incubated in m-TYH medium (TYH medium supplemented with 1mM caffeine and 1mM dibutyryl cyclic AMP), the frozen-thawed spermatozoa showed a good sperm survival rate and hyperactive movement after 30 min of incubation. A total of 59 germinal vesicle (GV) stage oocytes were collected from 4 ovaries of 4 female red-bellied tamarins regardless of their menstrual cycles. At 24, 48, and 72 h after incubation in TCM-199 medium containing 10% fetal calf serum (FCS), 30%, 53%, and 71% oocytes, respectively, either showed germinal vesicle breakdown (GVBD) or had extruded the first polar body. Although the oocytes matured in vitro were inseminated by frozen-thawed spermatozoa, no eggs became fertilized.
