G-Band Staining of Mouse Embryo Chromosomes by Urea Treatment - Journal of Mammalian Ova Research (JMOR)

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Abstract

Vol.13 No.1

G-Band Staining of Mouse Embryo Chromosomes by Urea Treatment

JMOR, 13(1) 44-47, 1996
DOI: 10.1274/jmor.13.44

Midori Yoshizawa* Wei Zhang Susumu Muramatsu

Department of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University, Utsunomiya 321, Japan

Chromosome preparations of mouse morulae and blastocysts derived from in-vitro fertilization were made by a new air-drying method, and then subjected to various methods of G-band staining. Excellent G-band figures of embryo chromosomes were obtained by a modified method of urea treatment. The G-band method is shown in the present paper.

Keywords: Chromosome G-band In-vitro fertilization Mouse embryo

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