Abstract
Vol.13 No.1
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Time Sequence of Meiotic Maturation in Cumulus-Enclosed and Denuded Porcine Oocytes Stimulated by Gonadotrophins, Estradiol-17β, Fetal Bovine Serum and Follicular Fluid -Synergistic Effect of Follicular Fluid with Estradiol-17β or Serum on the Expulsion of First Polar Body-
JMOR, 13(1) 12-18, 1996
DOI: 10.1274/jmor.13.12
DOI: 10.1274/jmor.13.12
This study was conducted to evaluate the effects of gonadotrophins (PMSG, hCG), fetal bovine serum (FBS), estradiol-17β (E2) and porcine follicular fluid (pFF) on the timing of nuclear maturation and the expulsion of first polar body in cumulus-enclosed (CC+) or denuded (CC-) porcine oocytes. CC+ and CC- oocytes were cultured in mTALP-PVA medium supplemented with the above stimulants alone or in combination for 24, 32 and 46 h. PMSG at all doses significantly (p<0.05) promoted the meiotic resumption (GVBD) in CC+ oocytes, compared to those in control. When CC+ were stimulated by hCG (15 IU/ml), FBS (15%) and E2 (10 μg) for 32 h, the percentage of GVBD was higher than that of control (p<0.05) and for 24 h culture oocytes (p<0.05). After 46 h of culture, PMSG, hCG and E2 significantly (p<0.05) promoted the expulsion of first polar body (Pb1) in CC+ than CC- oocytes. At the same time, pFF in the presence of E2 or FBS significantly (p<0.05) increased the expulsion of Pb1, compared to the stimulation by respective single stimulant. These results suggest that PMSG, hCG and E2 acted through the cumulus cells and stimulated the meiotic maturation; but FBS and pFF directly stimulate, and that pFF in combination with FBS or E2 showed a important role in promoting the expulsion of Pb1.
