Abstract
Vol.12 No.2
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Age-Related Changes of Lectin Bindings on the Cell Surface of Unfertilized Mouse Ova
JMOR, 12(2) 79-83, 1995
DOI: 10.1274/jmor.12.79
DOI: 10.1274/jmor.12.79
Lectin bindings on the surface of unfertilized mouse ova immediately after ovulation were histochemi-cally examined, and were compared among 30-day-old, 60- to 90-day-old, 180- to 210-day-old and 270-day-old mice. The bindings of PNA, GS-I, DBA, SBA, BPA, MPA, GS-II, WGA and Con A were observed from a weak to an intense degree on the cell surface of ova in mice from different age groups, but those of UEA-I and LPA were not. Although the strength in bindings of PNA, DBA, SBA, GS-II, BPA, WGA and Con A did not differ among the age groups, the strength of GS-I and MPA bindings intensified in 270-day-old mice. From the results, it may be said that the glycoconjugates on the cell surface of mouse ova immediately after ovulation contain galactose, N-acetylgalactosamine, N-acetylglucosamine and man-nose, and that the amount of galactose and N-acetylgalactosamine increases as the animals get older.
