Japanese society of Ova Research

Abstract

Vol.12 No.1

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Effect of Insulin and Basic Fibroblast Growth Factor (bFGF) on Bovine Embryo Development In Vitro in a Defined Medium after In Vitro Fertilization
JMOR, 12(1) 15-21, 1995
DOI: 10.1274/jmor.12.15
Yuji Takagi*1 Keizo Kobayashi2 Hiroyoshi Hoshi2 Hirotada Tsujii1 Shichiro Sugawara3 Taneaki Oikawa2 
1Faculty of Agriculture, Shinshu University, Minamiminowa, Kamiina-gun, Nagano 399-45, 2Research Institute for the Functional Peptides, Yamagata 990, and 3Faculty of Agriculture, Tohoku University, Sendai 981, Japan

Present experiments were carried out to evaluate positive effects of growth factors and conditioned medium (CM) from bovine granulosa cells (BGC) in a defined medium on bovine embryo development. In Experiment 1, insulin (19%; blastocyst / oocytes examined) and basic fibroblast growth factor (bFGF; 14%) individually stimulated embryonic development to blastocyst stage in a defined medium as effective as serum did (15%; p>0.05), but neither bovine lipoprotein (LP; 3%) nor bovine serum albumin (BSA; 1%) affected the stimulation of embryo development. In Experiment 2, denuded embryos did not traverse beyond 8- to 16-cell block without the co-culture with BGC, regardless of the addition of insulin and bFGF. In Experiment 3, serum-free, CM of BGC in the presence of insulin stimulated blastocyst formation (11%) as well as co-culture system (18%). These results suggest that insulin and bFGF stimulate early bovine embryonic development through the action of a certain embryotrophic factor produced by the activation of the physiological function of BGC.

Keywords: Cattle  Serum-free  Insulin  bFGF  Conditioned medium 
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