Abstract
Vol.32 No.3
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Brief Note
Transportation of preimplantation porcine embryos without cryopreservation using a novel embryo carrier
JMOR, 32(3) 121-127, 2015
DOI: 10.1274/jmor.32.121
DOI: 10.1274/jmor.32.121
The objective of this study was to develop a transportation system for fresh porcine embryos in a chemically defined medium without reducing their viability using a novel embryo carrier. When embryos were introduced in 0.25-ml straws with porcine blastocyst medium (PBM) and given a vibration load on a shaker for 20 h to mimic transportation conditions at 38 °C, there was no significant difference in survival rates between the vibration and no-vibration groups. Next, an embryo carrier was developed that can maintain interior conditions of 5% CO2 and 38 °C for the transportation of embryos. The embryos on Day 5 after insemination were divided into three groups. In the first group, the embryos were introduced to straws with PBM and the straws were sealed (sealed group). In the second group, the embryos were transported in a tube with a CO2 pouch (CO2 gas group). They were transported by a door-to-door delivery service. In the last group, the embryos were cultured in an incubator (not-transported group). Although the survival rate of embryos in the sealed group was significantly lower than that of not-transported group, the survival rates and the hatching rate of the embryos in the CO2 gas group were similar to those of not-transported group of embryos (100% vs 97.4% and 57.9% vs 47.5%, respectively). Our results demonstrate that in vivo-derived porcine embryos can be transported without harm in PBM using our carrier.
