Japanese society of Ova Research

Abstract

Vol.31 No.1

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Case Report
Successful Pregnancy Following Transfer of Frozen-thawed Day 7 Blastocysts Derived from Transported Oocytes
JMOR, 31(1) 52-56, 2014
DOI: 10.1274/jmor.31.52
1Ono Ladies Clinic, Hyogo 675-1375, Japan
2Department of Biophysics, Kobe University Graduate School of Health Sciences, Hyogo 654-0142, Japan
3Asagiri Hospital, Hyogo 673-0852, Japan

Purpose: Pelvic adhesions inhibit follicular growth and ovum transport, causing female infertility. In this report, we describe successful ovum pick-up (OPU) during adhesiotomy in a woman with severe adhesions that prevented transvaginal OPU. Subsequently, in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) were attempted after transport of cumulus-oocyte complexes (COCs). Materials and methods: Clinical examination revealed a chocolate cyst on the patient's left ovary. Her right ovary was adherent to the posterior part of the uterus. COCs were harvested during adhesiotomy and stored at 37°C in a 1.5-ml tube containing Sperm Washing Medium. The tube was transported to our clinic using hot gel bags and a portable infant incubator. Immediately upon arrival, IVF and ICSI were performed. On day 7, two ICSI-derived blastocysts were cryopreserved. Two months later, these blastocysts were transferred to the patient's uterus. Results: The patient conceived, and a normal, healthy boy was born. Conclusions: In summary, we performed OPU during adhesiotomy, and COCs were transported to the infertility clinic on the same day; subsequently, the patient was successfully impregnated using the transported oocytes.

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