Japanese society of Ova Research

Abstract

Vol.29 No.3

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Expression and Potential Role of GATA6 in Ruminant Trophoblasts during Peri-implantation Periods
JMOR, 29(3) 135-141, 2012
DOI: 10.1274/jmor.29.135
Hanako Bai14 Toshihiro Sakurai1 Atsushi Ideta2 Yoshito Aoyagi2 James D. Godkin3 Kazuhiko Imakawa1* 
1Laboratory of Animal Breeding, Graduate School of Agricultural and Life Science, the University of Tokyo, Tokyo 113-8657, Japan
2Zen-Noh ET Center, Hokkaido 080-1407, Japan
3Department of Animal Science, University of Tennessee, Knoxville, 37996 TN, USA
4Research Fellow of the Japan Society for the Promotion of Science (JSPS)

Expression of GATA6 has been found during embryonic development in many mammalian species. In mouse embryonic development, GATA6 is a primitive endoderm (PrE) marker. However, the expression and effect of GATA6 in ruminant ungulates has not been well characterized. In this report, the expression of GATA6 mRNA was examined in the uteri of ruminants. GATA6 mRNA was detected in days 17, 20, and 22 (day 0=day of estrus) bovine conceptuses and in days 15, 17, and 21 ovine conceptuses. In both cases, GATA6 mRNA increased on day 22 or 21 after conceptus attachment to the uterine epithelium. GATA6 mRNA was also detected in bovine trophoblast CT-1 or F3 cells and ovine trophoblast oTr cells. In transient transfection analyses using the upstream region of the bovine IFNT gene (bIFNT, IFN-tau-c1), GATA6 overexpression was effective in the up-regulation of the bIFNT construct that had been transfected into human choriocarcinoma JEG3 or bovine ear-derived fibroblast EF cells. The observation in which GATA6 increased IFNT transcription suggests that in addition to lineage specification, GATA6 may have acquired ruminant-specific functions involving the control of trophoblast gene expression.

Keywords: GATA6  Trophoblast cells  Implantation  Ruminants 
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