Abstract
Vol.29 No.1
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Original
Impact of the Volume of Cytoplasm Aspirated into the Injection Pipette at the Time of Oolemma Breakage on the Fertilization Rate after ICSI: A Preliminary Study
JMOR, 29(1) 82-87, 2012
DOI: 10.1274/jmor.29.82
DOI: 10.1274/jmor.29.82
Purpose: To investigate the impact of the volume of cytoplasm aspirated into the injection pipette on the fertilization, embryo development and implantation ability after intracytoplasmic sperm injection. Methods: This was a preliminary observational study conducted between October 2010 and December 2010. We divided oocytes into two groups based on the farthest point reached by the aspirated cytoplasm. The intersection of the ICSI pipette and the outer surface of the zona pellucida was used as a marker. When the farthest point reached by aspirated cytoplasm from the tip of the injection pipette was less than the marker, we classified the oocyte as group A, and when it was beyond the marker, we classified the oocyte as group B. Results: The oocyte degeneration rate of group A (7%) was higher than that of group B (3%), but the difference was not significant. The fertilization rate (91% versus 71%) was significantly higher in group A than in group B (P = 0.007). On the other hand, the rates of survival, cleavage, good quality day-3 embryo, good quality blastocyst and pregnancy following single day-3 embryo or day-5 embryo transfers were similar between the two groups. Conclusions: These results indicate that the volume of cytoplasm aspirated into the injection pipette affects the fertilization rate, but does not influence embryo development or implantation ability after intracytoplasmic sperm injection.
