Japanese society of Ova Research

Abstract

Vol.28 No.1

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Brief Note
Tumor Protein p53 Gene Polymorphism and Developmental Competence of Embryos Derived from in Vitro Fertilization in Old-aged and Long-term Infertile Japanese Black Cows
JMOR, 28(1) 75-78, 2011
DOI: 10.1274/jmor.28.75
Tomoko Yuzawa1,2 Emiko Fukui1,2 Hiromichi Matsumoto1,2 Tomohiro Kawada3 Akio Kawanobe4 Kazutaka Niira4 Masanori Ochi5 Midori Yoshizawa1,2* 
1United Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8059, Japan
2Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University, Utsunomiya 321-8505, Japan
3Tochigi Prefecture Livestock Experiment Station, Tochigi 321-3303, Japan
4Tochigi Prefecture Dairy Experimental Institute, Tochigi 329-2724, Japan
5Ochi Yume Clinic Nagoya, Nagoya 460-0002, Japan

The present study analyzed tumor protein p53 gene (TP53) polymorphism as a factor in uterine implantation and establishment of pregnancy in four old-aged Japanese Black cows with long-term infertility. They had G allele or A allele in exon 6 of the gene. Oocytes were collected from these females using ovum pick up (OPU) and fertilized in vitro. Only some of the oocytes from individuals having G allele had developmental competence to the blastocyst stage, while no oocytes having the A allele had the competence to develop to the blastocyst stage. In conclusion, we suggest that a cause of long-term infertility in old-aged Japanese Black cows may be a polymorphism of TP53 affecting the competence of embryonic development.

Keywords: Cow  Japanese Black  TP53  Infertile  In vitro fertilization 
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