The fate of human oocytes with impaired stretchability of the oolemma during piezo-ICSI was investigated in vitro (development into blastocysts in 5-day culture) and in vivo (implantation 4 weeks after embryo transfer). Oolemma of metaphase-II human oocytes was penetrated either before application of a piezo-pulse at stretching of less than 75% of the diameter (category-Low), or by a piezo-pulse when the oolemma was stretched over 75% of the diameter (category-High). Following in vitro culture, oocyte survival and developmental capacity to the blastocyst stage were compared between the two categories of oolemma stretchability. Moreover, we investigated whether oolemma stretchability was independently related as a risk factor of implantation using multivariate logistic regression. The post-injection survival rate of category-Low oocytes (73.6%) was significantly (P < 0.01) lower than that of category-High oocytes (99.4%). The blastocyst yield of category-Low oocytes (36.7%; calculated from surviving oocytes) was similar to that of category-High oocytes (44.5%). The low oolemma stretchability was found to be an independent risk factor of implantation (odds ratio 4.18, 95% confidence interval 1.04-16.74, P = 0.043). In conclusion, stretchability of human oolemma during piezo-ICSI affects post-injection survival, but not the developmental potential to blastocysts. Since oolemma stretchability also affects the implantation, we propose this parameter as a criterion for embryo selection in single embryo transfer.