Japanese society of Ova Research

Abstract

Vol.26 No.1

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Enhanced Oocyte Activation by Intracytoplasmic Injection of Porcine Spermatozoa Pre-treated with Dithiothreitol
JMOR, 26(1) 54-60, 2009
DOI: 10.1274/jmor.26.54
Hiroyuki Watanabe1,2* Junko Akiyama3 Mohammad Musharraf Uddin Bhuiyan1 Yutaka Fukui1 
1Department of Food Production Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
2Department of Animal Production Science, The United Graduate School of Agricultural Science, Iwate University, Morioka 020-8550, Japan
3Mizuuchi Women's Clinic, Asahikawa 078-8234, Japan

The aim of this study was to confirm the effect of dithiothreitol (DTT)-treated spermatozoa on oocyte activation following intracytoplasmic sperm injection (ICSI). Boar spermatozoa with or without DTT treatment (5 mM, 30 min) were injected into in vitro matured porcine oocytes, and the nuclear phase in presumptive zygotes was observed at 3 h intervals up to 12 h after ICSI. Furthermore, developmental competence of embryos produced by DTT-treated or non-treated spermatozoa was monitored after cultivation in vitro for 144 h. Male and female pronuclear formation rates in the oocytes injected with DTT-treated spermatozoa were significantly (P < 0.05) higher than those in the oocytes injected with non-treated spermatozoa. Additionally, we observed that female pronuclear formation was linked to male pronuclear formation. Sperm treatment with DTT improved (P < 0.05) subsequent development up to the blastocyst stage. These findings confirm the efficiency of DTT in in vitro porcine embryo production mediated by ICSI. We conclude that DTT treatment improves the formation of not only male pronuclei but also female pronuclei in porcine ICSI.

Keywords: Assisted reproductive technology  Dithiothreitol  Intracytoplasmic sperm injection  Oocyte activation 
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