Abstract
Vol.24 No.3
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Original
Effect of Ovary Storage on Development of Bovine Oocytes after Intracytoplasmic Sperm Injection, Parthenogenetic Activation, or Somatic Cell Nuclear Transfer
JMOR, 24(3) 114-119, 2007
DOI: 10.1274/jmor.24.114
DOI: 10.1274/jmor.24.114
The purpose of this study was to examine the effect of ovary storage on the development of bovine oocytes after intracytoplasmic sperm injection (ICSI), parthenogenetic activation, or somatic cell nuclear transfer (SCNT). Oocytes were obtained from ovaries stored in PBS for 2 to 6 h (control group) or 26 to 30 h (stored group) at 15°C. The maturation rate of the oocytes was significantly lower in the stored group (67%) than in the control group (78%). The degeneration rate of the oocytes was significantly higher in the stored group (24%) than in the control group (2%). ICSI and parthenogenetic oocytes from stored ovaries had a significantly decreased development to the blastocyst stage compared with the control (ICSI 8% vs. 24%, parthenogenetic activation 15% vs. 31%). However, the development rate to blastocysts of SCNT embryos derived from cumulus cells was not different between the two groups (38% vs. 38%). Also, the storage period of ovaries did not decrease the pregnancy rate of SCNT embryos, and cloned calves were produced in both groups with the same efficiency (21% vs. 21%). In summary, ovary storage at 15°C for 26 to 30 h reduced the maturation rate and in vitro development rate of bovine oocytes after ICSI or parthenogenetic activation, but did not decrease the blastocyst formation rate or survival rate after embryo transfer in SCNT.
