Handmade Somatic Cell Cloning and Related Studies in Farm Animals

Mini Review

JMOR, 24(3) 99-106, 2007
DOI: 10.1274/jmor.24.99

Takashi Nagai¹* Kazuhiro Kikuchi² Kei Imai³ Mokhamad Fahrudin⁴

¹National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki 305-0901, Japan
²Divison of Animal Sciences, Reproductive Biology Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan
³Department of Technology, National Livestock Breeding Center, Nishishirakawa, Fukushima 961-8511, Japan
⁴Department of Anatomy Physiology and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor 16680, Indonesia

The method of enucleating recipient oocytes by bisection with a metal-blade has been adapted to somatic cell nuclear transfer (SCNT) using zona-free mammalian oocytes, and the process named handmade cloning (HMC). Besides the simplification of enucleation without using a manipulator, this technique also provides for a reduction in the amount or the elimination of expensive equipment that is usually indispensable for conventional SCNT. In this review, we will highlight some recent studies on HMC and related studies in farm animals, especially cloning using enucleation by the gradient centrifugation of zona-free oocytes to produce a large number of recipient cytoplasts at once.

Keywords: Somatic cell nuclear transfer Hand made cloning Oocyte Enucleation

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Vol.24 No.3