Abstract
Vol.23 No.3
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Original
Effects of Sperm Extract and its Molecular Weight Fractions on Oocyte Activation in Miniature Pig Spermatozoa
JMOR, 23(3) 122-127, 2006
DOI: 10.1274/jmor.23.122
DOI: 10.1274/jmor.23.122
This study was conducted to compare the quantity of total protein in miniature pig sperm extract (SE) extracted by two methods [sonication and a method using Mammalian Protein Extraction Reagent (M-PER)]. Furthermore, the effects of injection with SE extracted by M-PER and fractionated SE on porcine oocyte activation were examined. The total amount of protein extracted from miniature pig spermatozoa (1 × 109 sperm) by M-PER was significantly (P<0.05) larger than that extracted by sonication. When the porcine oocyte activation rate after injection with SE extracted by M-PER at different protein concentrations was examined, the highest activation (31.4%) was induced by injection with 0.08 mg/ml SE, and the value was significantly (P<0.05) higher than the control (buffer injection). When the effect of injection with fractionated SE (>100 kDa and <100 kDa) was examined, the activation rates of the control (non-fraction), >100 kDa, and <100 kDa groups were 63.6, 48.7, and 46.4%, respectively. Significant differences (P<0.05) were observed between the control and <100 kDa groups. These results indicate that SE extracted by M-PER from miniature pig sperm is effective for porcine oocyte activation. Oocyte activation could be induced efficiently by injection with SE using M-PER. Both <100 kDa SE and >100 kDa SE contain effective materials for porcine oocyte activation.
