Production of Viable Porcine Embryos by In Vitro Fertilization (IVF) and Intracytoplasmic Sperm Injection (ICSI)

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Abstract

Vol.23 No.3

Mini Review

Production of Viable Porcine Embryos by In Vitro Fertilization (IVF) and Intracytoplasmic Sperm Injection (ICSI)

JMOR, 23(3) 96-106, 2006
DOI: 10.1274/jmor.23.96

Kazuhiro Kikuchi¹* Michiko Nakai¹ Arata Shimada² Naomi Kashiwazaki²

¹Division of Animal Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan
²Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Sagamihara 229-8501, Japan

In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are highly specialized procedures used for artificial production of successive generations of domestic animals. However, in pigs, the efficacy of these procedures in generating viable embryos is still poor. We focus here on both IVF and ICSI in pigs in relation to the physiology of fertilization (morphological changes and nuclear remodeling). This knowledge should contribute to the development of advanced technologies for in vitro production of zygotes that have the potential for full-term development to the offspring stage after transfer to recipients.

Keywords: Pig Fertilization Nuclear remodeling IVF ICSI

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