Human sperm cannot fertilize oocytes immediately upon ejaculation, but must acquire the ability to bind and penetrate the zona pellucida. Hyperactivation, which is linked to the process of capacitation, is a vigorous pattern of sperm motility marked by wide-amplitude, high-velocity, whiplash movements of the flagellum. This study was performed to investigate the correlation between hyperactivated (HA) motility patterns assessed by computer-aided sperm analysis (CASA) and the fertilization rate (FR) in vitro. Swim-up sperm were collected in 135 IVF cycles with at least 3 oocytes collected. Because no cases satisfied the HA motility pattern of “Star-spin”, patients were divided into 3 groups: Sperm with curvilinear velocity (VCL) ≥ 100 μM/sec, linearity (LIN) < 60% and amplitude of lateral head displacement (ALH) ≥ 5 μM were “All HA”. Sperm with straight-line velocity (VSL) ≥ 40 μM/sec, LIN ≥ 60% and ALH < 5 μM were “Non-HA”. Others were defined as “Transition phase”. The FRs in 81 “All HA” cycles, 33 “Non-HA” cycles, and 21 “Transition phase” cycles were 79.5 ± 26.6%, 65.4 ± 32.5%, and 80.8 ± 27.3% respectively. There was a significant difference between “All HA” and “Non-HA” cycles in the FRs (P=0.018). In 27 (20.0%) of 135 IVF cycles, the FRs were ≤ 50% (“poor” group). Eleven (13.6%) of 81 “All HA” cycles, 12 (36.3%) of 33 “Non-HA” cycles, and 4 of 21 “Transition phase” cycles belonged to the “poor” group. There was a significant difference between “All HA” and “Non-HA” cycles (P=0.006) in these incidences. The better FRs were obtained in patients with “All HA” cycles, and lower FRs were obtained in those with “Non-HA” cycles. These findings suggest that the assessment of HA motility patterns by means of CASA could be one of the predictors of human sperm fertilizing ability.