Abstract
Vol.19 No.3
- « prev
- ↑Table of Contents
- next »
Original
Evaluation of Equine Oocytes from Preserved Ovaries Using Intracytoplasmic Sperm Injection
JMOR, 19(3) 71-76, 2002
DOI: 10.1274/jmor.19.71
DOI: 10.1274/jmor.19.71
This study was conducted to examine if oocytes obtained from one day preserved equine ovaries could be used for IVM and IVF research. Ovaries were transported at 20°C over a long distance from a slaughterhouse to a laboratory taking about 24 h. Oocytes with compact cumulus cell layers were collected from ovaries and cultured for 42 h in IVMD101 medium at 38.5°C in 5% CO2 in air. The proportions of metaphase-II stage oocytes increased significantly between 24 and 30 h of culture and reached a plateau at 30 h. After 30 h of culture, the denuded oocytes with a first polar body were subjected to intracytoplasmic sperm injection (ICSI) using frozen-thawed stallion spermatozoa. The ICSI oocytes were activated by 10 min treatment with ionophore A23187 and 6 h treatment with cycloheximide, and cultured for up to 10 days in CR1aa medium. The proportion of ICSI oocytes fertilized normally (defined as those with 2 pronuclei and 2 polar bodies) was 44%. In vitro culture of ICSI oocytes resulted in a cleavage rate of 52% and a blastocyst development rate of 9%. These results indicate that equine oocytes derived from one day preserved ovaries can reach metaphase-II stage during 30 h of IVM, and that IVM oocytes can develop into blastocysts after ICSI.
