Japanese society of Ova Research

Abstract

Vol.19 No.1

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Original
Partial Incision of the Zona Pellucida using Piezo-micromanipulator Improves In Vitro Fertilization using Low-motility Spermatozoa of Infertile Transgenic Mice
JMOR, 19(1) 26-31, 2002
DOI: 10.1274/jmor.19.26
Yosuke Kawase12* Nobuo Kamada1 Hiroshi Suzuki1, 3 
1Chugai Pharmaceutical Co., Ltd. and
2Chugai Research Institute for Medical Science, Inc., 1-135, Komakado, Gotemba, 412-8513 Japan,
3National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13, Inada, Obihiro, 080-8555 Japan

To overcome infertility of male transgenic mice with low-motility spermatozoa, we examined the benefits of the zona pellucida incision using a piezo-micromanipulator (ZIP) on in vitro fertilization (IVF) using low-motility spermatozoa. The incision was about 26 µm long, representing 8% of the perimeter of the zona pellucida of the mature oocyte. Over 95% of cumulus-free oocytes survived after ZIP. The in vitro fertilization rate of ZIP oocytes by low-motility spermatozoa of the transgenic mice was 22-57%, compared with 1-2% of cumulus-intact oocytes. After embryo transfer of ZIP zygotes at 2-cell stage into the oviducts of recipients, 11-20% of the transferred embryos developed to term. Our results indicate that ZIP is a useful technique for IVF of ova by low-motile spermatozoa and subsequent embryo transfer.

Keywords: ART  Embryo transfer  Infertility  IVF  Mice 
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