Abstract
Vol.43 No.1
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Original
Effects of supplementation of growth factors to in vitro maturation medium on early embryonic development and gene expression in bovine in vitro fertilized embryos
JMOR, 43(1) 53-59, 2026
Bovine in vitro fertilization (IVF) technology is valuable for the efficient production of quality calves. However, bovine IVF embryos have problems such as low embryonic development and conception rates and frequent occurrence of large-offspring syndrome due to abnormal gene expression. The addition of FLI (FGF2, LIF, and IGF1) to in vitro maturation (IVM) medium improves oocyte maturation. Therefore, this study examined the effects of FLI addition to IVM medium on the maturation of oocytes, and preimplantation development, and the gene expression status of bovine embryos after IVF. There was no significant difference in the nuclear maturation rates of oocytes matured in IVM medium with or without FLI supplementation. The blastocyst developmental rate and mRNA expression levels (FGF4, IGFBP-2, OCT-4, CDX2, and AQP3) showed no differences between maturation in IVM medium with or without FLI supplementation. In conclusion, our results show that different IVM media and the addition of FLI did not affect the nuclear maturation of bovine oocytes, preimplantation development or gene expression status of bovine IVF embryos.
