Japanese society of Ova Research

Abstract

Vol.43 No.1

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Brief Note
In vitro development of bovine embryos using a two-step culture method
JMOR, 43(1) 47-51, 2026
Shunpei Oguma1,2 Ryotaro Miura3 Hiroshi Tajimi4 Aya Iwaki1 Kana Hasegawa1 Erina Sasaki1 Konosuke Okada1 Kenji Furui2 Hitoshi Ushijima1* 
1Department of Applied Life Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan, 2Clinic MAMA, Imajuku 3-34-1, Ogaki, Gifu 503-0807, Japan, 3Department of Veterinary, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan, 4Tajimi Bovine ET Clinic, Higashitabaru 645-1, Seki, Gifu 501-3921, Japan

We examined a two-step culture method for bovine in vitro embryo production (IVEP). Modified synthetic oviduct fluid without glucose (mSOF) was used for early-cleavage-stage embryos, followed by 5% fetal calf serum (FCS) supplemented with SOF for morula-stage embryos. When SOF+FCS was used for the latter half of culture, the blastocyst development rate was significantly higher (P < 0.05) than in culture in mSOF for the full 7 days after IVF. When TCM199 supplemented with 5.54 mM glucose, FCS, and 0.1 mM β-mercaptoethanol was used for the latter half of the culture there was no significant difference in the blastocyst rate from that of SOF+FCS, but at 6 and 9 days after IVEP, the cell numbers of embryos were significantly greater in the TCM199 supplemented medium. These results show that glucose in the medium is important for embryonic development and that switching to an energy-rich medium at the morula stage is beneficial for the production of high-quality embryos.

Keywords: Culture medium for bovine embryos  In vitro production  SOF  Total cell number  Two-step culture method 
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