Abstract
Vol.43 No.1
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Chemicals for improving the viability of vitrified-warmed bovine oocytes
JMOR, 43(1) 33-38, 2026
Unfertilized bovine oocytes are rich in cryo-sensitive cytoplasmic lipid droplets and less permeable to water and CPA due to their spherical shape, which has the smallest surface to volume ratio, making cryopreservation difficult by the conventional freeze-thawing procedure. Over the last few decades, applying innovative vitrification-warming procedures, characterized by ultra-rapid cooling in a very limited amount of cryoprotective solution (MVC vitrification), has improved oocyte cryopreservation, enabling high quality blastocyst production after IVF/ICSI of warmed oocytes. However, the developmental competence of vitrified-warmed bovine oocytes still needs to be improved. In this article, the efficiencies of bovine blastocyst production from warmed mature M-II-stage oocytes are listed as a function of cryodevice types in the MVC vitrification. Furthermore, low MW chemicals (L-carnitine, resveratrol, α-tocopherol, ROCK inhibitor Y-27632, etc.), which have been reported to improve warmed oocyte viability in some domestic species, are highlighted for their structure, timing of oocyte treatment, and extent of viability improvement.
