Abstract
Vol.42 No.2
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Successful Live Births Following Artificial Oocyte Activation Using A23187 Combined with Puromycin in Cases of Fertilization Failure
JMOR, 42(2) 89-95, 2025
Intracytoplasmic sperm injection (ICSI) is a widely adopted treatment for couples facing severe male infertility or fertilization failure. However, fertilization failure occurs in 1–5% of ICSI cases, with oocyte activation failure being one of the primary causes. To address this issue, artificial oocyte activation (AOA) using calcium ionophores such as A23187 is commonly employed. Nonetheless, the efficacy of this protocol is limited in certain cases. In two cases where AOA with A23187 alone failed to improve the fertilization rate, a combination of A23187 and puromycin improved the fertilization rate and facilitated successful live births following embryo transfer. These results suggest that AOA with A23187 and puromycin may serve as an effective treatment option for cases unresponsive to conventional methods.
