
Recently, due to an increase in ICSI cases, the Piezo-ICSI method has attracted increasing attention. In addition to not requiring cytoplasm aspiration, Piezo-ICSI can be controlled by various machines, minimizing the differences in skills of embryologists. It is also expected to improve the normal fertilization rates of ICSI and reduce the rate of degeneration after ICSI. Our clinic introduced Piezo-ICSI in 2011 and now performs Piezo-ICSI in almost all clinical cases. In this review, I would like to discuss Piezo-ICSI based on the establishment of the technique and clinical outcomes at our clinic, as well as our learning experiences with mouse Piezo-ICSI, which were the technique is essential for the establishment of this technique.