Clinical practice of embryo biopsy for preimplantation genetic testing for aneuploidy in assisted reproductive technology

Content

Abstract

Vol.42 No.1

Clinical practice of embryo biopsy for preimplantation genetic testing for aneuploidy in assisted reproductive technology

JMOR, 42(1) 23-29, 2025

Hiroyuki Tomari* Katsuko Kunitake Kie Kuboyama Keiko Uchimura Hitomi Simose Haruka Tsukigawa Anna Kitaoka Hazuki Seki Yukina Kitamura Mayu Nagata Saki Gondo Yukako Sano Kensuke Saito Chisato Kodera Kou Honjo Yumi Nagata

Center for Reproductive Medicine, IVF Nagata Clinic, 6F 1-12-1 Tenjin, Chuo-ku, Fukuoka 810-0001, Japan

Preimplantation genetic testing (PGT-A) is becoming increasingly popular worldwide, and is expected to become more widespread in Japan in the future. Biopsy is an invasive technique for the embryo and must be performed with extreme caution as it may affect the outcome of PGT-A and/or embryo viability. The usefulness of biopsy targets such as polar bodies, blastomeres, and trophectoderm (TE) has been reported, and TE biopsies are currently the mainstream. TE biopsy requires many steps to master, including prebiopsy preparation, opening of the zona pellucida of the blastocyst, excision of TE cells, and tubing, and technicians must be well trained.

Keywords: Assisted reproductive technology Preimplantation genetic testing for aneuploidy Blastocyst Trophectoderm biopsy Training for biopsy

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