Practice of trophectoderm biopsy at our clinic - Journal of Mammalian Ova Research (JMOR)

Practice of trophectoderm biopsy at our clinic

JMOR, 42(1) 9-14, 2025

Yamato Mizobe¹* Yukari Kuwatsuru^1,2 Yuko Kuroki¹ Yumiko Fukumoto¹ Mari Tokudome¹ Harue Moewaki¹ Satoha Aihoshi¹ Kiyoka Uenohori² Yuji Orita^1,2 Tokiko Iwakawa^1,2 Kazuhiro Takeuchi^1,2

¹Takeuchi Ladies Clinic/Center for Reproductive Medicine, 502-2 Higashimochida, Aira, Kagoshima 899-5421, Japan, ²Takeuchi Ladies Clinic/ART Kagoshima Branch, Kirameki Nangoku Bld. 3F, 43-20 Kouraicho, Kagoshima 890-0051, Japan

Currently, embryo biopsy is mainly performed by trophectoderm (TE) biopsy. TE biopsy involves various steps such as embryo culture, embryo sorting, TE cell harvesting, tubing, storage of harvested cells, and blastocyst freezing after TE cell harvesting. For TE cell collection, we use the pulling-stretching method, in which cells are cut by laser irradiation, and a biopsy is performed without prior zona opening. In this method, the zona is opened simultaneously at the time of TE biopsy. The biopsy technique is an essential skill for embryologists and intracytoplasmic sperm injection; therefore, an accurate diagnosis may not be possible if the biopsy technique is unstable. The biopsy technique should be selected based on the merits and demerits of the various available methods.

Keywords: Preimplantation genetic testing for aneuploidy Pulling-stretching method Trophectoderm biopsy

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Vol.42 No.1