Abstract
Vol.41 No.2
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Visualization of cross-contamination during freezing and thawing of sperm and embryos using fluorescent samples
JMOR, 41(2) 69-74, 2024
Potential sources of cross-contamination were examined after using mouthpiece pipettes and embryo/sperm freezing devices. A fluorescent fluid was used as a tracer and detected with black light. Mouthpiece pipetting resulted in traces of the fluid on the pipettes, the freezing devices, the culture dishes, the stereo microscope's stage and focus knobs, the incubator door, the hands and clothes of the operator, and the hands of another person who touched the contaminated area. The freezing straws showed traces of the fluid on their exterior, the hands of the operator performing the freezing and thawing, the straw sealer, the freezing stage, and the semen straw cutter. The fluid remained on the straw cutter even after wiping it with alcohol cotton. The fluid on the Makler counting chamber was only removed by soaking it in 0.1% sodium hypochlorite disinfectant solution for 1 hour, not by rinsing it with water. Technicians should follow standard precautions and the WHO manual provides standard laboratory methods for semen analysis.
