Abstract
Vol.41 No.1
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Original
The area of oocyte cytoplasmic shrinkage can be used to predict fertilization of oocytes after ICSI
JMOR, 41(1) 35-44, 2024
We examined whether oocyte cytoplasmic shrinkage after ICSI can be used for early prediction of fertilization. This was a retrospective study of 164 two pronuclei (2PN) zygotes, 19 abnormal zygotes (11 1PN and 8 ≥3PN) and 26 unfertilized (0PN) oocytes in 120 cycles which were observed using a time-lapse system. Image collection began 30 min after ICSI, and measurements of oocyte cytoplasmic area and diameter were made 13 times over the course of 5.3 h. Following ICSI, cytoplasmic areas shrank significantly more between <0.5–0.9 h (98.8 ± 1.4%) (mean ± SD) and <4.9–5.3 h (92.2 ± 2.6%) in 2PN zygotes than they did in 0PN oocytes (99.8 ± 1.2%, 97.7±1.9%; P < 0.01). Similar to 2PN zygotes, the shrinkage rates of the cytoplasmic areas declined over time in both 1PN and ≥3PN zygotes. However, the normality or abnormality of fertilization could not be distinguished by the measurement. The area under the ROC curve and optimal cutoff value for the prediction of fertilization were 0.947 and 97.120%, respectively, at <2.9–3.3 h after ICSI. Oocyte cytoplasmic shrinkage had no significant effect on embryo development. Measurement of oocyte cytoplasmic shrinkage after ICSI can confirm fertilization within 5.3 h with a fairly high accuracy and can be a useful criterion for fertilization prediction.
