Abstract
Vol.37 No.1
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Effect of resveratrol pre-treatment on mitochondrial number and developmental ability of bovine vitrified-warmed embryos
JMOR, 37(1) 23-30, 2020
Cryopreservation induces mitochondrial dysfunctions and resveratrol induces mitochondrial biogenesis. The present study examined the effect of resveratrol treatment before vitrification on the developmental ability and mitochondrial kinetics of bovine embryos. Treatment of early developmental stage embryos with resveratrol for 24 h increased the levels of SIRT1 expression but did not affect embryo development to the blastocyst stage or the mitochondrial DNA copy number in the blastocyst. Vitrification of embryos (2 days post fertilization) reduced the developmental ability and mitochondrial number of embryos compared with their respective values in fresh embryos, but the pretreatment of embryos with resveratrol restored them. In addition, resveratrol pretreatment increased the amount of extracellular mitochondrial DNA released into the culture medium. Furthermore, resveratrol pretreatment reduced the levels of dsDNA and TOMM20 in embryos 1 day after thawing. In conclusion, resveratrol treatment before vitrification effectively improved embryonic development and increased mitochondrial numbers in the resultant blastocysts.
