Abstract
Vol.37 No.1
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Maturity classification and clinical application of oocytes with abnormal zona pellucida
JMOR, 37(1) 37-42, 2020
Oocyte maturation is usually determined by the presence or absence of the first polar body, but there are cases in which maturation determination is prevented by morphological abnormality. Six cases who underwent oocyte retrieval at our clinic had common features such as indented zona pellucida and narrow perivitelline spaces. Many oocytes derived from these cases were judged immature oocytes without the presence of polar bodies, and insemination was not performed. Immunofluorescence staining of spindles and chromosomes, in some of the oocytes collected from these 6 cases that were judged to be immature, revealed that some of the oocytes had matured to the MII stage. Based on this result, oocyte maturation was determined using a spindle visualization device and insemination of MII oocytes was performed. Fertilized oocytes with two pronuclei were obtained, and after embryo transfer, two infants were successfully delivered. For cases where it is difficult to determine maturity due to morphological abnormalities of oocytes, a larger number of mature oocytes can be obtained by changing the determination method.
