Japanese society of Ova Research

Abstract

Vol.37 No.1

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In vitro spermatogenesis using chemically defined media
JMOR, 37(1) 17-21, 2020
Hiroyuki Sanjo1 Takehiko Ogawa2* 
1Department of Urology, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan, 2Laboratory of Biopharmaceutical and Regenerative Sciences, Institute of Molecular Medicine and Life Science, Yokohama City University Association of Medical Science, 3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan

We reported in 2011 the successful induction and completion of mouse spermatogenesis by culturing immature testis tissues using a culture medium comprising α-minimum essential medium (α-MEM) supplemented with 4% AlbuMAX, bovine serum albumin purified by chromatography. Sperm and spermatids obtained in that culture were used for micro-insemination, resulting in the production of healthy offspring. However, the ingredients in AlbuMAX which played a role in inducing spermatogenesis were not clear. In order to identify the important factors for spermatogenesis, chemically-defined media (CDM) were formulated. CDM containing retinoic acid, hormones (LH, FSH, T, T3), and lipids including free fatty acids, cholesterol, phosphatidylcholine, and sphingomyelin, were competent in inducing spermatogenesis. In this short review, we discuss factors and substances necessary for spermatogenesis.

Keywords: Spermatogenesis  Testis  Organ culture  Retinoic Acid  Testosterone 
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